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human fetal osteoblastic cell line hfob1 19  (ATCC)


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    ATCC human fetal osteoblastic cell line hfob1 19
    Human Fetal Osteoblastic Cell Line Hfob1 19, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fetal+osteoblast+cell+line/hFOB+1%2E19/pm42286305-60-17-27
    Average 98 stars, based on 813 article reviews
    human fetal osteoblastic cell line hfob1 19 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Formation of 3D Human Osteoblast Spheroids Incorporating Extracellular Matrix-Mimetic Phage Peptides as a Surrogate Bone Tissue Model.
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19) used in this study was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA, catalog number CRL-11372; Cellosaurus database accession CVCL_3708). hFOB 1.19 cells were cultured in a 1:1 mixture of Ham’s F12 Medium and Dulbecco’s Modified Eagle Medium (D8437, Sigma, Life Science, Gillingham, UK), supplemented with 2.5 mM L-glutamine (G7513), 0.3 mg/mL G418 (4727878001, Merck Life Science, Milan, Italy), 10% fetal bovine serum (F7524), and 1% penicillin/streptomycin/amphotericin (P4333). ..

    Article Title: Crosslinked Gelatin‐Polycaprolactone Scaffolds: Balancing Biocompatibility and Inflammatory Response for Tissue Engineering Applications
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19 ATCC CRL11372) was cultured in Dulbecco's Modified Eagle Medium: Nutrient Mixture F12, supplemented with 10% FBS and an antibiotic solution containing 1000 IU/mL of penicillin, 100 μg/ mL of streptomycin, and 0.3 μg/mL of fungizone. ..

    Article Title: Synthesis and Characterization of Ibuprofen-TiO 2 Functionalized PCL Biomembranes as Candidate Materials for Wound Dressing Applications.
    Article Snippet: Additionally, crystalline phase analysis was conducted by X-ray (XRD) using a diffractometer operating in Bragg–Brentano geometry with Cu-Kα lamp (λ = 1.5417 Å) over a range of 2θ = 5◦ to 2θ = 40◦. .. The human fetal osteoblast cell line (hFOB, ATCC CRL-11372) was used for biological evaluations. hFOB cells were cultured in 75 cm2 culture flasks with Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (FBS, Biosciences, Princeton, NJ, USA), 2.5 mM L-glutamine, and antibiotic solution (streptomycin 100 μg/mL and penicillin 100 U/mL, Sigma-Aldrich). ..

    Article Title: Development of injectable cuttlebone derived nanohydroxyapatite hydrogel for osteoblast cell encapsulation
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19) (Cat No. CRL-11372, ATCC, USA) was cultured in a growth medium for cell expansion and an osteogenic medium to stimulate osteogenic differentiation. ..

    Article Title: Synthesis and Characterization of Ibuprofen–TiO 2 Functionalized PCL Biomembranes as Candidate Materials for Wound Dressing Applications
    Article Snippet: Additionally, crystalline phase analysis was conducted by X-ray (XRD) using a diffractometer operating in Bragg–Brentano geometry with Cu-Kα lamp (λ = 1.5417 Å) over a range of 2θ = 5° to 2θ = 40°. .. The human fetal osteoblast cell line (hFOB, ATCC CRL-11372) was used for biological evaluations. hFOB cells were cultured in 75 cm 2 culture flasks with Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (FBS, Biosciences, Princeton, NJ, USA), 2.5 mM L-glutamine, and antibiotic solution (streptomycin 100 μg/mL and penicillin 100 U/mL, Sigma-Aldrich). ..

    Article Title: Formation of 3D Human Osteoblast Spheroids Incorporating Extracellular Matrix-Mimetic Phage Peptides as a Surrogate Bone Tissue Model
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19) used in this study was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA, catalog number CRL-11372; Cellosaurus database accession CVCL_3708). hFOB 1.19 cells were cultured in a 1:1 mixture of Ham’s F12 Medium and Dulbecco’s Modified Eagle Medium (D8437, Sigma, Life Science, Gillingham, UK), supplemented with 2.5 mM L-glutamine (G7513), 0.3 mg/mL G418 (4727878001, Merck Life Science, Milan, Italy), 10% fetal bovine serum (F7524), and 1% penicillin/streptomycin/amphotericin (P4333). ..

    Modification:

    Article Title: Formation of 3D Human Osteoblast Spheroids Incorporating Extracellular Matrix-Mimetic Phage Peptides as a Surrogate Bone Tissue Model.
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19) used in this study was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA, catalog number CRL-11372; Cellosaurus database accession CVCL_3708). hFOB 1.19 cells were cultured in a 1:1 mixture of Ham’s F12 Medium and Dulbecco’s Modified Eagle Medium (D8437, Sigma, Life Science, Gillingham, UK), supplemented with 2.5 mM L-glutamine (G7513), 0.3 mg/mL G418 (4727878001, Merck Life Science, Milan, Italy), 10% fetal bovine serum (F7524), and 1% penicillin/streptomycin/amphotericin (P4333). ..

    Article Title: Crosslinked Gelatin‐Polycaprolactone Scaffolds: Balancing Biocompatibility and Inflammatory Response for Tissue Engineering Applications
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19 ATCC CRL11372) was cultured in Dulbecco's Modified Eagle Medium: Nutrient Mixture F12, supplemented with 10% FBS and an antibiotic solution containing 1000 IU/mL of penicillin, 100 μg/ mL of streptomycin, and 0.3 μg/mL of fungizone. ..

    Article Title: Synthesis and Characterization of Ibuprofen-TiO 2 Functionalized PCL Biomembranes as Candidate Materials for Wound Dressing Applications.
    Article Snippet: Additionally, crystalline phase analysis was conducted by X-ray (XRD) using a diffractometer operating in Bragg–Brentano geometry with Cu-Kα lamp (λ = 1.5417 Å) over a range of 2θ = 5◦ to 2θ = 40◦. .. The human fetal osteoblast cell line (hFOB, ATCC CRL-11372) was used for biological evaluations. hFOB cells were cultured in 75 cm2 culture flasks with Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (FBS, Biosciences, Princeton, NJ, USA), 2.5 mM L-glutamine, and antibiotic solution (streptomycin 100 μg/mL and penicillin 100 U/mL, Sigma-Aldrich). ..

    Article Title: Synthesis and Characterization of Ibuprofen–TiO 2 Functionalized PCL Biomembranes as Candidate Materials for Wound Dressing Applications
    Article Snippet: Additionally, crystalline phase analysis was conducted by X-ray (XRD) using a diffractometer operating in Bragg–Brentano geometry with Cu-Kα lamp (λ = 1.5417 Å) over a range of 2θ = 5° to 2θ = 40°. .. The human fetal osteoblast cell line (hFOB, ATCC CRL-11372) was used for biological evaluations. hFOB cells were cultured in 75 cm 2 culture flasks with Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (FBS, Biosciences, Princeton, NJ, USA), 2.5 mM L-glutamine, and antibiotic solution (streptomycin 100 μg/mL and penicillin 100 U/mL, Sigma-Aldrich). ..

    Article Title: Formation of 3D Human Osteoblast Spheroids Incorporating Extracellular Matrix-Mimetic Phage Peptides as a Surrogate Bone Tissue Model
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19) used in this study was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA, catalog number CRL-11372; Cellosaurus database accession CVCL_3708). hFOB 1.19 cells were cultured in a 1:1 mixture of Ham’s F12 Medium and Dulbecco’s Modified Eagle Medium (D8437, Sigma, Life Science, Gillingham, UK), supplemented with 2.5 mM L-glutamine (G7513), 0.3 mg/mL G418 (4727878001, Merck Life Science, Milan, Italy), 10% fetal bovine serum (F7524), and 1% penicillin/streptomycin/amphotericin (P4333). ..



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    human fetal osteoblastic cell line hfob1 19  (ATCC)
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    Adhesion of hFOB 1.19 cells on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale modifications. Cell adhesion after 4 h ( A ) and 24 h ( B ) is shown relative to tissue culture plastic (TCP), which was set to 100%. Meso-scale modified surfaces (Mod-80, Mod-120, Mod-160) generally exhibited significantly higher cell adhesion compared with unmodified zirconia for both 3Y and 5Y materials, with differences becoming more pronounced after 24 h. A reduction in cell adhesion from 4 h to 24 h was observed mainly on unmodified and Mod-80 and Mod-120 3Y surfaces, whereas Mod-160 3Y and 5Y surfaces sustained cell adhesion over time. Significant differences were also detected between specific meso-scale feature sizes, indicating an influence of meso-scale structural design on the maintenance of cell adhesion. Significant differences between groups are indicated by square brackets; for clarity, asterisks denoting statistical significance were omitted. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 9.
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    Adhesion of hFOB 1.19 cells on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale modifications. Cell adhesion after 4 h ( A ) and 24 h ( B ) is shown relative to tissue culture plastic (TCP), which was set to 100%. Meso-scale modified surfaces (Mod-80, Mod-120, Mod-160) generally exhibited significantly higher cell adhesion compared with unmodified zirconia for both 3Y and 5Y materials, with differences becoming more pronounced after 24 h. A reduction in cell adhesion from 4 h to 24 h was observed mainly on unmodified and Mod-80 and Mod-120 3Y surfaces, whereas Mod-160 3Y and 5Y surfaces sustained cell adhesion over time. Significant differences were also detected between specific meso-scale feature sizes, indicating an influence of meso-scale structural design on the maintenance of cell adhesion. Significant differences between groups are indicated by square brackets; for clarity, asterisks denoting statistical significance were omitted. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 9.

    Journal: Bioengineering

    Article Title: Meso-Scale Modifications in Additively Manufactured Zirconia: Topographical Design and Its Influence on Cell–Material Interactions

    doi: 10.3390/bioengineering13050498

    Figure Lengend Snippet: Adhesion of hFOB 1.19 cells on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale modifications. Cell adhesion after 4 h ( A ) and 24 h ( B ) is shown relative to tissue culture plastic (TCP), which was set to 100%. Meso-scale modified surfaces (Mod-80, Mod-120, Mod-160) generally exhibited significantly higher cell adhesion compared with unmodified zirconia for both 3Y and 5Y materials, with differences becoming more pronounced after 24 h. A reduction in cell adhesion from 4 h to 24 h was observed mainly on unmodified and Mod-80 and Mod-120 3Y surfaces, whereas Mod-160 3Y and 5Y surfaces sustained cell adhesion over time. Significant differences were also detected between specific meso-scale feature sizes, indicating an influence of meso-scale structural design on the maintenance of cell adhesion. Significant differences between groups are indicated by square brackets; for clarity, asterisks denoting statistical significance were omitted. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 9.

    Article Snippet: The experimental procedures intend the analysis of cell–material interactions utilized the human fetal osteoblast cell line hFOB 1.19 (CRL-11372; American Type Culture Collection, ATCC ® , Manassas, VA, USA).

    Techniques: Modification

    Proliferation of hFOB 1.19 cells on DLP-printed 3Y ( A ) and 5Y ( B ) zirconia surfaces with and without meso-scale structural modifications. Cell numbers were determined after 4 h (day 0) and daily for a total of 4 days. Growth curves are shown for unmodified and meso-scale structurally modified surfaces (Mod-80, Mod-120, Mod-160) and compared with cells cultured on standard tissue culture plastic (control). No significant differences in proliferation rates, assessed by comparison of growth curve slopes, were observed between surface modifications, zirconia materials, or the control, indicating comparable cell growth on all tested surfaces.

    Journal: Bioengineering

    Article Title: Meso-Scale Modifications in Additively Manufactured Zirconia: Topographical Design and Its Influence on Cell–Material Interactions

    doi: 10.3390/bioengineering13050498

    Figure Lengend Snippet: Proliferation of hFOB 1.19 cells on DLP-printed 3Y ( A ) and 5Y ( B ) zirconia surfaces with and without meso-scale structural modifications. Cell numbers were determined after 4 h (day 0) and daily for a total of 4 days. Growth curves are shown for unmodified and meso-scale structurally modified surfaces (Mod-80, Mod-120, Mod-160) and compared with cells cultured on standard tissue culture plastic (control). No significant differences in proliferation rates, assessed by comparison of growth curve slopes, were observed between surface modifications, zirconia materials, or the control, indicating comparable cell growth on all tested surfaces.

    Article Snippet: The experimental procedures intend the analysis of cell–material interactions utilized the human fetal osteoblast cell line hFOB 1.19 (CRL-11372; American Type Culture Collection, ATCC ® , Manassas, VA, USA).

    Techniques: Modification, Cell Culture, Control, Comparison

    Osteogenic differentiation-associated gene expression of hFOB 1.19 cells cultured on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale structural modifications. Cells were analyzed after 1, 2, and 7 days. Relative mRNA expression levels of early (RUNX2, ( A )), intermediate (ALPL, ( B ); COL1A1, ( C )), and late (BGLAP, ( D )) osteogenic markers were quantified by qPCR. Meso-scale structural modifications (Mod-80, Mod-120 and Mod-160) induced material- and feature size-dependent differences in gene expression, with pronounced early and sustained induction of BGLAP on modified 3Y zirconia, particularly on Mod-160, whereas 5Y zirconia showed more moderate or absent differentiation-related responses. Significant differences between groups are indicated by square brackets; asterisks were omitted for clarity. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 3.

    Journal: Bioengineering

    Article Title: Meso-Scale Modifications in Additively Manufactured Zirconia: Topographical Design and Its Influence on Cell–Material Interactions

    doi: 10.3390/bioengineering13050498

    Figure Lengend Snippet: Osteogenic differentiation-associated gene expression of hFOB 1.19 cells cultured on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale structural modifications. Cells were analyzed after 1, 2, and 7 days. Relative mRNA expression levels of early (RUNX2, ( A )), intermediate (ALPL, ( B ); COL1A1, ( C )), and late (BGLAP, ( D )) osteogenic markers were quantified by qPCR. Meso-scale structural modifications (Mod-80, Mod-120 and Mod-160) induced material- and feature size-dependent differences in gene expression, with pronounced early and sustained induction of BGLAP on modified 3Y zirconia, particularly on Mod-160, whereas 5Y zirconia showed more moderate or absent differentiation-related responses. Significant differences between groups are indicated by square brackets; asterisks were omitted for clarity. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 3.

    Article Snippet: The experimental procedures intend the analysis of cell–material interactions utilized the human fetal osteoblast cell line hFOB 1.19 (CRL-11372; American Type Culture Collection, ATCC ® , Manassas, VA, USA).

    Techniques: Gene Expression, Cell Culture, Expressing, Modification