Journal: Bioengineering
Article Title: Meso-Scale Modifications in Additively Manufactured Zirconia: Topographical Design and Its Influence on Cell–Material Interactions
doi: 10.3390/bioengineering13050498
Figure Lengend Snippet: Osteogenic differentiation-associated gene expression of hFOB 1.19 cells cultured on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale structural modifications. Cells were analyzed after 1, 2, and 7 days. Relative mRNA expression levels of early (RUNX2, ( A )), intermediate (ALPL, ( B ); COL1A1, ( C )), and late (BGLAP, ( D )) osteogenic markers were quantified by qPCR. Meso-scale structural modifications (Mod-80, Mod-120 and Mod-160) induced material- and feature size-dependent differences in gene expression, with pronounced early and sustained induction of BGLAP on modified 3Y zirconia, particularly on Mod-160, whereas 5Y zirconia showed more moderate or absent differentiation-related responses. Significant differences between groups are indicated by square brackets; asterisks were omitted for clarity. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 3.
Article Snippet: The experimental procedures intend the analysis of cell–material interactions utilized the human fetal osteoblast cell line hFOB 1.19 (CRL-11372; American Type Culture Collection, ATCC ® , Manassas, VA, USA).
Techniques: Gene Expression, Cell Culture, Expressing, Modification